Journal: International Journal of Molecular Sciences

Article Title: High-Content Imaging and Machine Learning Classify Phenotypical Change in Coronary Artery Endothelial Cells Caused by BPS

doi: 10.3390/ijms27073259

Figure Lengend Snippet: Representative high-content microscopy images of human coronary artery endothelial cells (HCAEC) exposed to vehicle control (CTRL) or 0.1 µM Bisphenol S (BPS) for 96 h and stained using the PhenoVue Cell Painting assay. For each condition, a representative field acquired at 40× magnification and a higher-magnification inset are shown. Rows correspond to the individual fluorescence channels: Hoechst 33342 (nuclei), PhenoVue Fluor 488 Concanavalin A (endoplasmic reticulum and intracellular membranes), PhenoVue 512 nucleic acid stain (RNA/nucleoli), PhenoVue Fluor 555 wheat germ agglutinin (plasma membrane), PhenoVue 641 mitochondrial stain (mitochondria), and the merged image. White boxes represent the part of the image used for the related inset. Scale bar: 50 µm, 40× objective.

Article Snippet: Primary human coronary artery endothelial cells (HCAEC; ATCC ® PCS-100-020TM, Innovation, VA, USA) were cultured according to the supplier’s recommendations.

Techniques: Microscopy, Control, Staining, Fluorescence, Clinical Proteomics, Membrane

Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial attachment in vitro. A) Representative images of SEM and F‐actin staining. B) HCAEC attachment and C) spreading on ePTFE, ePTFE with passively bound PF8 (ePTFE‐PF8), PPN‐coated ePTFE (PPN), and PPN‐PF8 functionalized ePTFE, n = 3 per sample. Scale bar = 300 µm. * p < 0.05, *** p < 0.001 versus ePTFE.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial proliferation in vitro. A) Representative images of SEM and F‐actin staining of HCAEC proliferation on days 1 and 3. B) HCAEC proliferation and C) spreading on ePTFE, ePTFE with passively bound PF8, PPN, and PPN‐PF8 functionalized ePTFE. * p < 0.05, *** p < 0.001 versus ePTFE on each day, Scale bar = 300 µm. n = 3 per sample.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Circulation. Cardiovascular interventions

Article Title: Impact of stent surface on thrombogenicity and vascular healing: a comparative analysis of metallic and polymeric surfaces.

doi: 10.1161/CIRCINTERVENTIONS.113.000120

Figure Lengend Snippet: Figure 4. Endothelialization of uncoated and polyvinylidene flu- oride-co-hexafluoropropene (PVDF-HFP)–coated platinum chro- mium (PtCr) stents. A, Absorbance values of CD31 expression (minus background) for endothelial cells (ECs) covering stents at 7 (n=9) and 14 days (n=7). At 7 (P<0.01) and 14 (P=0.02) days, there were significant differences between the endothelialization between uncoated and PVDF-HFP–coated PtCr stents. Values shown are mean±SEM . B, Human coronary artery EC coverage on PVDF-HFP at 7 (n=9) and 14 days (n=7) was normalized to the respective value for PtCr at each time point. Endothelialization of uncoated PtCr stents was greater at 7 and 14 days compared with that on PVDF-HFP–coated stents (P<0.01). Values shown are mean with 95% confidence interval. *Significantly different from PtCr at the respective time point (P<0.05).

Article Snippet: Endothelial cell (EC) growth on uncoated and PVDF-HFP–coated PtCr stents (3.0 mm×16 mm, ELEMENT) was evaluated using human coronary artery ECs (HCAECs; Cell Applications Inc, San Diego, CA), as previously described,11 and is described in detail in the online-only Data Supplement.

Techniques: Expressing

Journal: Circulation. Cardiovascular interventions

Article Title: Impact of stent surface on thrombogenicity and vascular healing: a comparative analysis of metallic and polymeric surfaces.

doi: 10.1161/CIRCINTERVENTIONS.113.000120

Figure Lengend Snippet: Figure 5. Representative micrographs of immunohistochemical staining of human coronary artery endothelial cells (HCAECs) on uncoated and polyvinylidene fluoride-co-hexafluoropropene (PVDF-HFP)–coated platinum chromium (PtCr) stents at 14 days. 4',6-diamidino-2-phenylindole (DAPI)–stained nuclei of HCAEC on (A) uncoated and (D) PVDF-HFP–coated stents demonstrate presence of cells at 14 days with greater levels on uncoated stents. Expression of vascular endothelial (VE)-cadherin at intracellular junctions of HCAECs on (B) uncoated stents is readily evident and reflects typical cobblestone morphology of endothelial cells (within circle). In contrast, VE-cadherin expression on HCAEC covering (D) PVDF-HFP–coated stents is relatively weaker at intracellular junctions (within circle), sug- gesting reduced barrier function compared with HCAEC covering uncoated stents. For comparison on uncoated PtCr stents, (H) an isotype control for VE-cadherin displays nominal back- ground staining. C, F, and I, DAPI and VE-cadherin images were merged with the transmitted images of the underlying stents to represent strut location.

Article Snippet: Endothelial cell (EC) growth on uncoated and PVDF-HFP–coated PtCr stents (3.0 mm×16 mm, ELEMENT) was evaluated using human coronary artery ECs (HCAECs; Cell Applications Inc, San Diego, CA), as previously described,11 and is described in detail in the online-only Data Supplement.

Techniques: Immunohistochemical staining, Staining, Expressing, Comparison, Control

Journal: Journal of Translational Medicine

Article Title: Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic

doi: 10.1186/s12967-024-05120-y

Figure Lengend Snippet: QA improved TMAO-induced inflammatory lesions and endothelial dysfunction in HCAECs. (A) CCK-8 was applied to detect the toxicity of QA on HCAECs. (B) CCK-8 was used to detect HCAECs proliferation. (C) The expression of COX-2, IL-6, E-selectin, ICAM-1, HMGB1 was detected by RT-qPCR. (D) The expression of p-P65, p-MAPK14 protein was detected by western blot. (E) HMGB1 levels were detected by ELISA. (F) The expression of ZO-2, VE-Cadherin and Occludin were detected by western blot. * P < 0.05 vs. Control, # P < 0.05 vs. TMAO

Article Snippet: To investigate the cytotoxicity of QA, human coronary artery endothelial cells (HCAECs, HUM-iCell-c006, iCell) were treated with 1, 2.5, 5, 10 and 20 μM QA.

Techniques: CCK-8 Assay, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Control